Recombinant DNA

In the Recombinant DNA technology the desired DNA fragment is isolated from the suitable cell of an organisms and is joined with a DNA fragment of some other organisms. In this technique the enzyme restriction endonuclease obtained from E.coli is used for cutting the DNA strands in both the organisms and the enxyme ligase cutting from E.coli is used for joining these DNA fragments with each other. Such DNA fragments can be obtained from two individuals of the same or different species. The new DNA fragment, so obtained by recombinant DNA technique, is known as hybrid DNA or recombinant DNA or gene. Such a hybrid gene can be introduced in the E.coli. A large number of copies of such DNA are formed as a result of the growth and multiplication of the host bacteria. Such bacteria having hybrid gene can be utilized to obtain specific types of genetical products (protein). The artificial production of insulin is now possible by this technique.