Assessing an Engineered enzyme as a biocatalyst

We have engineered an enzyme, tyrosinase, to exhibit higher ctivity towardsD-
tyrosine. The product of the reaction is D-DOPA a potential alternative drug for
the treatment of Parkinson’s disease (which is currently treated using
L-DOPA). We wish to assess the ability of this enzyme to produce D-DOPA and other catechol products through the development of abioprocess. protein Analysis of the substrate and product using High performance liquid chromatography

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