“Kintetics of a Single Substrate Enzyme Catalysed Reaction. The Hydrolysis of P-Nitrophenyl Phosphate by Mucosal Alkaline Phosphatase.”

ABSTRACT:

The applicability of both the Michealis-Menten equation and the Linweaver Burk plot have been applied in the following. Looking at both the rate of reaction of alkaline

phosphotase with and without the presence of an inhibitor (sodium phosphate).

1. Introduction:

Kinetics is the study of the rate of reaction in biochemistry involving enzymes. The rate of reaction can very from enzyme to enzyme, with each individual enzyme having an optimum unto which they work to their maximum potential and capability [1]. For the past century, the rate of reaction of enzyme kinetics has been greatly based on the work of Michealis and Menten and in particular the Michealis-Menten equation [2].

Rates of reaction can be manipulated and adjusted in a variety of ways, including temperature [2]. An increase in temperature will increase the rate and efficiency of the reaction until a…(short extract)