+1 862 207 3288 Aim/Hypothesis:
Show how PCR and specifically designed primers are used to amplify a small section of the human -globin gene to detect sickle cell anaemia.
Abstract:
Primers were made in various programs to be used in the polymerase chain reaction (PCR) for detection of the sickle cell anaemia mutation on chromosome eleven, codon six. A polymerase chain reaction was performed to detect a mutation on codon six. These results were then run using gel electrophoresis to show a mutation on codon six causing there to be 1 band of DNA shown as the restriction enzyme couldnt cut the primer due to the mutation and the coding not being correct for that enzyme to
recognize.
Introduction:
Sickle cell anaemia is the disease the affects red blood cells. The hemoglobin molecule has two parts; alpha () and beta (). Those with sickle cell disease have a mutation on chromosome 11, which…(short extract)
